Overview

Last Updated:  July 8, 2004.

UPDATE:

Due to lack of funding, we will not be able to ship seeds from the University of Wisconsin after July 15, 2004.  Our seed collection will be transferred to the ABRC at Ohio State University after July 15, 2004.  Seed distribution will be handled through the ABRC starting some time in the late summer or early fall of 2004.

The Knockout Facility at the University of Wisconsin has generated a new population of T-DNA transformed Arabidopsis lines and is currently sequencing the flanking DNA from individual lines in order to precisely map the locations of the T-DNA inserts.  The people who developed this new resource are listed here.

This new population was created using a T-DNA vector that carries a Ds Transposon Launch-pad and Cre/Lox recombination sites.  See below for more information on how these features can be used to knockout tandemly duplicated gene families or to knockout a single gene that has escaped mutagenesis in the existing populations.

        Ecotype:                   Col-0

        Number of lines:    63,000

        Seed archive:         Pools of one

        T-DNA Vector:        pDS-Lox

        Insert locations:    Shown on Salk T-DNA Express web site

        Seed requests:      Click here to request seeds

Unique features of this population

This new population can be used to knockout a gene that has not been hit in existing collections of T-DNA mutants.  Click here to learn more about using the Ds launch-pad present in our population for this purpose.

This new population can be also be used to generate targeted deletions of the Arabidopsis genome.  This type of targeted deletion could be used to knockout all of the members of group of genes present as a tandem array.   Click here to learn more about using the Cre/Lox system present in our population for this purpose.

Timeline for Sequencing

Our current funding will allow us to deposit ca. 10,000 flanking sequences in the Salk T-DNA Express web site by May 1, 2004.  If we obtain additional funding for this project then we will be able to complete sequencing of the entire population within the year.