Annotated sequence of pDs-Lox

Construction notes:  pDS-Lox was constructed using pCambia1300 as the plasmid backbone. The T-DNA region of pCambia1300 was replaced with the T-DNA region from pED204. The resulting plasmid was then modified by installing the Basta resistance gene from pSKI015 in place of the Kanamycin resistance marker found in pED204. Finally, the mutant Lox43 site present in pED204 was converted to a wild-type LoxP site.

The complete sequence of the T-DNA portion of pDs-Lox is provided below.
 

Position: 1 - 240
Feature: Vector sequences from pCambia-1300

Position: 241 - 266
Feature: T-DNA Left Border

Position: 316 - 523
Feature: Cauliflower Mosaic Virus 35S PolyA

Position: 1,729 - 2,146
Feature: Cauliflower Mosaic Virus 35S Promoter

Position: 2,150 - 2,183
Feature: Wild-type LoxP Site

Position: 2,196 - 2,206
Feature: Ds element 11 base-pair repeat

Position: 2,196 - 2,445
Feature: Ds element transposon sequence

Position: 2,447 - 3,115
Feature: Mannopine synthase promoter

Position: 3,116 - 3,752
Feature: mRNA sequence for Bar gene that provides Basta resistance in plants

Position: 3,195 - 3,746
Feature: Protein coding sequence for Bar gene that provides Basta resistance in plants

Position: 3,753 - 4,484
Feature: PolyA region from the Octopine Synthase gene.

Position: 4,516 - 5,027
Feature: Nopaline Synthase gene 3’ region.

Position: 5,046 - 5,079
Feature: Wild-type LoxP Site

Position: 5,082 - 6,964
Feature: mRNA encoding Luciferase

Position: 5,163 - 6,815
Feature: Protein coding sequence for Luciferase

Position: 6,965 - 7,300
Feature: Ds element transposon sequence

Position: 7,290 - 7,300
Feature: Ds element 11 base-pair repeat

Position: 7,333 - 8,671
Feature: mRNA for Hygromycin resistance gene. (plant selectable marker).

Position: 7,356 - 8,378
Feature: Protein coding sequence for Hygromycin resistance gene

Position: 8,684 - 8,941
Feature: Transcriptional terminator from Nopaline Synthase gene.

Position: 9,170 - 9,194
Feature: T-DNA Right Border
 
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GCTGATCACAGGCAGCAACGCTCTGTCATCGTTACAATCAACATGCTACCCTCCGCGAG
ATCATCCGTGTTTCAAACCCGGCAGCTTAGTTGCCGTTCTTCCGAATAGCATCGGTAAC
ATGAGCAAAGTCTGCCGCCTTACAACGGCTCTCCCGCTGACGCCGTCCCGGACTGATGG
GCTGCCTGTATCGAGTGGTGATTTTGTGCCGAGCTGCCGGTCGGGGAGCTGTTGGCTGG
CTGGTGGCAGGATATATTGTGGTGTAAACAAATTGACGCTTAGACAACTTAATAACACA
TTGCGGACGTTTTTAATGTACTGAATTAACGCCGAATTAATTCGGGGGATCTGGATTTT
AGTACTGGATTTTGGTTTTAGGAATTAGAAATTTTATTGATAGAAGTATTTTACAAATA
CAAATACATACTAAGGGTTTCTTATATGCTCAACACATGAGCGAAACCCTATAGGAACC
CTAATTCCCTTATCTGGGAACTACTCACACATTATTATGGAGAAACTCGAGGAGCTTAG
CAAGAGCTAACGGCTCTTCGCAACAAGGAGACCTCCCTCTCCACCGTGAAACACCGGTA
AAAGAAGAAGCAGTTCATTCTGCACTGGCCACTTTTACGCCAACTCAAGTAAAGGCTAT
TCCAGAGCAAACGGCTCCTGGTAAAGAATCAACAAATCCGTTGATGGCTAGTATCTTGC
CAAAAGATATGAACCCAGTTCAAACTGGGATAAGGCTTGCAGTGCCAGGGGACTTTTTA
CGTCCTCATCAGGGAATTCCAATCCCACAAAAATCTGAGCTTAGCAGCATAGTTGCTCC
TCTCAGAGCAGAATCGGGTATTCAACACCCTCATATCAACTACTACGTTGTGTATAACG
GTCCACACGCCGGTATATACGATGACTGGGGTTGTACAAAGGCGGCAACAAACGGCGTT
CCCGGAGTTGCACACAAGAAGTTTGCCACTATTACAGAGGCAAGAGCAGCAGCTGACGC
AAACAGTCGACTCTAGACTTAATTAAGGATCCGGCGCGTACACAACAAGTCAGCAAACA
GACAGGTTGAACTTCATCCCCAAAGGAGAAGCTCAACTCAAGCCCAAGAGCTTTGCGAA
GGCCTTAACCAGCCCACCAAAGCAAAAAGCCCACTGGCTCACGCTAGGAACCAAAAGGC
CCAGCAGTGATCCAGCCCCAAAAGAGATCTCCTTTGCCCCGGAGATCACCATGGACGAC
TTTCTCTATCTCTACGATCTAGGAAGAAAGTTCGACGGAGAAGGTGACGATACCATGTT
CACCACCGATAATGAGAAGATTAGCCTCTTCAATTTCAGAAAGAATGCTGACCCACAGA
TGGTTAGAGAGGCCTACGCGGCAGGTCTCATCAAGACGATCTACCCGAGTAATAATCTC
CAGGAGATCAAATACCTTCCCAAGAAGGTTAAAGATGCAGTCAAAAGATTCAGGACTAA
CTGCATCAAGAACACAGAGAAAGATATATTTCTCAAGATCAGAAGTACTATTCCAGTAT
GGACGATTCAAGGCTTGCTTCATAAACCAAGGCAAGTAATAGAGATTGGAGTCTCTAAG
AAAGTAGTTCCTACTGAATCAAAGGCCATGGAGTCAAAAATTCAGATCGAGGATCTAAC
AGAACTCGCCGTGAAGACTGGCGAACAGTTCATACAGAGTCTTTTACGACTCAATGACA
AGAAGAAAATCTTCGTCAACATGGTGGAGCACGACACTCTCGTCTACTCCAAGAATATC
AAAGATACAGTCTCAGAAGACCAAAGGGCTATTGAGACTTTTCAACAAAGGGTAATATC
GGGAAACCTCCTCGGATTCCATTGCCCAGCTATCTGTCACTTCATCAAAAGGACAGTAG
AAAAGGAAGGTGGCACCTACAAATGCCATCATTGCGATAAAGGAAAGGCTATCGTTCAA
GATGCCTCTGCCGACAGTGGTCCCAAAGATGGACCCCCACCCACGAGGAGCATCGTGGA
AAAAGAAGACGTTCCAACCACGTCTTCAAAGCAAGTGGATTGATGTGATATCTCCACTG
ACGTAAGGGATGACGCACAATCCCACTATCCTTCGCAAGACCCTTCCTCTATATAAGGA
AGTTCATTTCATTTGGAGAGGATCCATAACTTCGTATAGCATACATTATACGAAGTTAT
TAGCTCTCCATTCAGGGATGAAAGTAGGATGGGAAAATCCCGTACCGACCGTTATCGTA
TAACCGATTTTGTTAGTTTTATCCCGATCGATTTCGAACCCGAGGTAAAAAACGAAAAC
GGAACGGAAACGGGATATACAAAACGGTAAACGGAAACGGAAACGGTAGAGCTAGTTTC
CCGACCGTTACACCGGGATCCCGTTTTTAATCGGGATGATCCCGTTTCGTTACCGTATT
TTCTAATTCGGGATGACTGCAATATGGGCATTGAGACCGATGTTCGTTCCGGAACCTTG
CACGCCCCAGAGCTTCTCACCGTTCACGACAATTTCCTTCTCGTTGAGGTCGGTCGCGC
CATGTCGGATGAAATAAAAACTTTTGATACCAGCGGGGGCCTTCGCAGAGCCGAGGTAG
GTCTGAGAAATTGGCATTTTCACGTGTGGAAGATATGAATTTTTTTGAGAAACTAGATA
AGATTAATGAATATCGGTGTTTTGGTTTTTTCTTGTGGCCGTCTTTGTTTATATTGAGA
TTTTTCAAATCAGTGCGCAAGACGTGACGTAAGTATCCGAGTCAGTTTTTATTTTTCTA
CTAATTTGGTCGTTTATTTCGGCGTGTAGGACATGGCAACCGGGCCTGAATTTCGCGGG
TATTCTGTTTCTATTCCAACTTTTTCTTGATCCGCAGCCATTAACGACTTTTGAATAGA
TACGCTGACACGCCAAGCCTCGCTAGTCAAAAGTGTACCAAACAACGCTTTACAGCAAG
AACGGAATGCGCGTGACGCTCGCGGTGACGCCATTTCGCCTTTTCAGAAATGGATAAAT
AGCCTTGCTTCCTATTATATCTTCCCAAATTACCAATACATTACACTAGCATCTGAATT
TCATAACCAATCTCGATACACCAAATCGACTCTAGCGAATTCCTCGAGTATAAGAGCTC
TATTTTTACAACAATTACCAACAACAACAAACAACAAACAACATTACAATTACTATTTA
CAATTACCATGAGCCCAGAACGACGCCCGGCCGACATCCGCCGTGCCACCGAGGCGGAC
ATGCCGGCGGTCTGCACCATCGTCAACCACTACATCGAGACAAGCACGGTCAACTTCCG
TACCGAGCCGCAGGAACCGCAGGAGTGGACGGACGACCTCGTCCGTCTGCGGGAGCGCT
ATCCCTGGCTCGTCGCCGAGGTGGACGGCGAGGTCGCCGGCATCGCCTACGCGGGCCCC
TGGAAGGCACGCAACGCCTACGACTGGACGGCCGAGTCAACCGTGTACGTCTCCCCCCG
CCACCAGCGGACGGGACTGGGCTCCACGCTCTACACCCACCTGCTGAAGTCCCTGGAGG
CACAGGGCTTCAAGAGCGTGGTCGCTGTCATCGGGCTGCCCAACGACCCGAGCGTGCGC
ATGCACGAGGCGCTCGGATATGCCCCCCGCGGCATGCTGCGGGCGGCCGGCTTCAAGCA
CGGGAACTGGCATGACGTGGGTTTCTGGCAGCTGGACTTCAGCCTGCCGGTACCGCCCC
GTCCGGTCCTGCCCGTCACCGAGATTTGATGACCCGGGGGATCGATCCTAGAGTCCTGC
TTTAATGAGATATGCGAGACGCCTATGATCGCATGATATTTGCTTTCAATTCTGTTGTG
CACGTTGTAAAAAACCTGAGCATGTGTAGCTCAGATCCTTACCGCCGGTTTCGGTTCAT
TCTAATGAATATATCACCCGTTACTATCGTATTTTTATGAATAATATTCTCCGTTCAAT
TTACTGATTGTACCCTACTACTTATATGTACAATATTAAAATGAAAACAATATATTGTG
CTGAATAGGTTTATAGCGACATCTATGATAGAGCGCCACAATAACAAACAATTGCGTTT
TATTATTACAAATCCAATTTTAAAAAAAGCGGCAGAACCGGTCAAACCTAAAAGACTGA
TTACATAAATCTTATTCAAATTTCAAAAGTGCCCCAGGGGCTAGTATCTACGACACACC
GAGCGGCGAACTAATAACGCTCACTGAAGGGAACTCCGGTTCCCCGCCGGCGCGCATGG
GTGAGATTCCTTGAAGTTGAGTATTGGCCGTCCGCTCTACCGAAAGTTACGGGCACCAT
TCAACCCGGTCCAGCACGGCGGCCGGGTAACCGACTTGCTGCCCCGAGAATTATGCAGC
ATTTTTTTGGTGTATGTGGGCCCCAAATGAAGTGCAGGTCAAACCTTGACAGTGACGAC
AAATCGTTGGGCGGGTCCAGGGCGAATTTTGCGACAACATGTCGAGGCTCAGCAGGACC
TGCAGGCATGCAAGCTTATCGATATCTAGATCTTATCATATAATTTCTGTTGAATTACG
TTAAGCATGTAATAATTAACATGTAATGCATGACGTTATTTATGAGATGGGTTTTTATG
ATTAGAGTCCCGCAATTATACATTTAATACGCGATAGAAAACAAAATATAGCGCGCAAA
CTAGGATAAATTATCGCGCGCGGTGTCATCTATGTTACTAGATCGATCAAACTTCGGCA
CTGTGTAATGACGATGAGCAATCGAGAGGCTGACTAACAAAAGGTATGCCCAAAAACAA
CCTCTCCAAACTGTTTCGAATTGGAAGTTTCTGCTCATGCCGACAGGCATAACTTAGAT
ATTCGCGGGCTATTCCCACTAATTCGTCCTGCTGGTTTGCGCCAAGATAAATCAGTGCA
TCTCCTTACAAGTTCCTCTGTCTTGTGAAATGAACTGCTGACTGCCCCCCAAGAAAGCC
TCCTCATCTCCCAGTTGGCGGCGGCTGATACACCATCGAAAACCCACGTCCGAACACTT
GATACATGTGCCTGAGAAATAGGGTACCTAATAACTTCGTATAGCATACATTATACGAA
GTTATATGGATCCGAGCTTGGAATTCCTTTGTGTTACATTCTTGAATGTCGCTCGCAGT
GACATTAGCATTCCGGTACTGTTGGTAAAATGGAAGACGCCAAAAACATAAAGAAAGGC
CCGGCGCCATTCTATCCTCTAGAGGATGGAACCGCTGGAGAGCAACTGCATAAGGCTAT
GAAGAGATACGCCCTGGTTCCTGGAACAATTGCTTTTACAGATGCACATATCGAGGTGA
ACATCACGTACGCGGAATACTTCGAAATGTCCGTTCGGTTGGCAGAAGCTATGAAACGA
TATGGGCTGAATACAAATCACAGAATCGTCGTATGCAGTGAAAACTCTCTTCAATTCTT
TATGCCGGTGTTGGGCGCGTTATTTATCGGAGTTGCAGTTGCGCCCGCGAACGACATTT
ATAATGAACGTGAATTGCTCAACAGTATGAACATTTCGCAGCCTACCGTAGTGTTTGTT
TCCAAAAAGGGGTTGCAAAAAATTTTGAACGTGCAAAAAAAATTACCAATAATCCAGAA
AATTATTATCATGGATTCTAAAACGGATTACCAGGGATTTCAGTCGATGTACACGTTCG
TCACATCTCATCTACCTCCCGGTTTTAATGAATACGATTTTGTACCAGAGTCCTTTGAT
CGTGACAAAACAATTGCACTGATAATGAATTCCTCTGGATCTACTGGGTTACCTAAGGG
TGTGGCCCTTCCGCATAGAACTGCCTGCGTCAGATTCTCGCATGCCAGAGATCCTATTT
TTGGCAATCAAATCATTCCGGATACTGCGATTTTAAGTGTTGTTCCATTCCATCACGGT
TTTGGAATGTTTACTACACTCGGATATTTGATATGTGGATTTCGAGTCGTCTTAATGTA
TAGATTTGAAGAAGAGCTGTTTTTACGATCCCTTCAGGATTACAAAATTCAAAGTGCGT
TGCTAGTACCAACCCTATTTTCATTCTTCGCCAAAAGCACTCTGATTGACAAATACGAT
TTATCTAATTTACACGAAATTGCTTCTGGGGGCGCACCTCTTTCGAAAGAAGTCGGGGA
AGCGGTTGCAAAACGCTTCCATCTTCCAGGGATACGACAAGGATATGGGCTCACTGAGA
CTACATCAGCTATTCTGATTACACCCGAGGGGGATGATAAACCGGGCGCGGTCGGTAAA
GTTGTTCCATTTTTTGAAGCGAAGGTTGTGGATCTGGATACCGGGAAAACGCTGGGCGT
TAATCAGAGAGGCGAATTATGTGTCAGAGGACCTATGATTATGTCCGGTTATGTAAACA
ATCCGGAAGCGACCAACGCCTTGATTGACAAGGATGGATGGCTACATTCTGGAGACATA
GCTTACTGGGACGAAGACGAACACTTCTTCATAGTTGACCGCTTGAAGTCTTTAATTAA
ATACAAAGGATATCAGGTGGCCCCCGCTGAATTGGAATCGATATTGTTACAACACCCCA
ACATCTTCGACGCGGGCGTGGCAGGTCTTCCCGACGATGACGCCGGTGAACTTCCCGCC
GCCGTTGTTGTTTTGGAGCACGGAAAGACGATGACGGAAAAAGAGATCGTGGATTACGT
CGCCAGTCAAGTAACAACCGCGAAAAAGTTGCGCGGAGGAGTTGTGTTTGTGGACGAAG
TACCGAAAGGTCTTACCGGAAAACTCGACGCAAGAAAAATCAGAGAGATCCTCATAAAG
GCCAAGAAGGGCGGAAAGTCCAAATTGTAAAATGTAACTGTATTCAGCGATGACGAAAT
TCTTAGCTATTGTAATATTATATGCAAATTGATGAATGGTAATTTTGTAATTGTGGGTC
ACTGTACTATTTTAACGAATAATAAAATCAGGTATAGGTAACTAAAAAGGAATTCGAGC
TCAAGACTTATCACTTATGTGCTACATTAAACTATGTGTGCTCCAGATTTATATGGATT
TTATCTATGTTTAATTAAGACTTGTGTTTACAATTTTTTATATTTGTTTTTAAGTTTTG
AATATATGTTTTCATGTGTGATTTTACCGAACAAAAATACCGGTTCCCGTCCGATTTCG
ACTTTAACCCGACCGGATCGTATCGGTTTTCGATTACCGTATTTATCCCGTTCGTTTTC
GTTACCGGTATATCCCGTTTTCGTTTCCGTCCCGCAAGTTAAATATGAAAATGAAAACG
GTAGAGGTATTTTACCGACTGTTACCGACCGTTTTCATCCCTATCTCCATTGCTAGCCG
ATCCCCGGGAGCTTGGCGCTCGTCCGGGGGCAATCAGATATGAAAAAGCCTGAACTCAC
CGCGACGTCTGTCGAGAAGTTTCTGATCGAAAAGTTCGACAGCGTCTCCGACCTGATGC
AGCTCTCGGAGGGCGAAGAATCTCGTGCTTTCAGCTTCGATGTAGGAGGGCGTGGATAT
GTCCTGCGGGTAAATAGCTGCGCCGATGGTTTCTACAAAGATCGTTATGTTTATCGGCA
CTTTGCATCGGCCGCGCTCCCGATTCCGGAAGTGCTTGACATTGGGGAATTCAGCGAGA
GCCTGACCTATTGCATCTCCCGCCGTGCACAGGGTGTCACGTTGCAAGACCTGCCTGAA
ACCGAACTGCCCGCTGTTCTGCAGCCGGTCGCGGAGGCCATGGATGCGATCGCTGCGGC
CGATCTTAGCCAGACGAGCGGGTTCGGCCCATTCGGACCGCAAGGAATCGGTCAATACA
CTACATGGCGTGATTTCATATGCGCGATTGCTGATCCCCATGTGTATCACTGGCAAACT
GTGATGGACGACACCGTCAGTGCGTCCGTCGCGCAGGCTCTCGATGAGCTGATGCTTTG
GGCCGAGGACTGCCCCGAAGTCCGGCACCTCGTGCACGCGGATTTCGGCTCCAACAATG
TCCTGACGGACAATGGCCGCATAACAGCGGTCATTGACTGGAGCGAGGCGATGTTCGGG
GATTCCCAATACGAGGTCGCCAACATCTTCTTCTGGAGGCCGTGGTTGGCTTGTATGGA
GCAGCAGACGCGCTACTTCGAGCGGAGGCATCCGGAGCTTGCAGGATCGCCGCGGCTCC
GGGCGTATATGCTCCGCATTGGTCTTGACCAACTCTATCAGAGCTTGGTTGACGGCAAT
TTCGATGATGCAGCTTGGGCGCAGGGTCGATGCGACGCAATCGTCCGATCCGGAGCCGG
GACTGTCGGGCGTACACAAATCGCCCGCAGAAGCGCGGCCGTCTGGACCGATGGCTGTG
TAGAAGTACTCGCCGATAGTGGAAACCGACGCCCCAGCACTCGTCCGAGGGCAAAGGAA
TAGAGTAGATGCCGACCGAACAAGAGCTGATTTCGAGAACGCCTCAGCCAGCAACTCGC
GCGAGCCTAGCAAGGCAAATGCGAGAGAACGGCCTTACGCTTGGTGGCACAGTTCTCGT
CCACAGTTCGCTAAGCTCGCTCGGCTGGGTCGCGGGAGGGCCGGTCGCAGTGATTCAGG
CCCTTCTGGATTGTGTTGGTCCCCAGGGCACGATTGTCATGCCCACGCACTCGGGTGAT
CTGACTGATCCCGCAGATTGGAGATCGCCGCCCGTGCCTGCCGATTGGGTGCAGATCCG
TCGACCTGCAGATCGTTCAAACATTTGGCAATAAAGTTTCTTAAGATTGAATCCTGTTG
CCGGTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTAAGCATGTAATAATT
AACATGTAATGCATGACGTTATTTATGAGATGGGTTTTTATGATTAGAGTCCCGCAATT
ATACATTTAATACGCGATAGAAAACAAAATATAGCGCGCAAACTAGGATAAATTATCGC
GCGCGGTGTCATCTATGTTACTAGATCTCTAGAAGCTTGGCACTGGCCGTCGTTTTACA
ACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCC
CTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTG
CGCAGCCTGAATGGCGAATGCTAGAGCAGCTTGAGCTTGGATCAGATTGTCGTTTCCCG
CCTTCAGTTTAAACTATCAGTGTTTGACAGGATATATTGGCGGGTAAACCTAAGAGAAA
AGAGCGTTTATTAGAATAATCGGATATTTAAAAGGGCGTGAAAAGGTTTATCCGTTCGT
CCATTTGTATGTGCATGCCAACCACAGGGTTCCCCTCGGGATCAAAGTACTTTGATCCA
ACCCCTCCGCTGCTATAGTGCAGTCGGCTTCTGACGTTCAGTGCAGCCGTCTTCTGAAA
ACGACATGTCGCACAAGTCCTAAGTTACGCGACAGGCTGCCGCCCTGCCCTTTTCCTGG
CGTTTTCTTGTCGCGTGTTTTAGTCGCATAAAGTAGAATACTTGCGACTAGAACCGGAG
ACATTACGCCATGAACAAGAGCGCCGCCGCTGGCCTGCTGGGCTATGCCCGCGTCAGCA
CCGACGACCAGGACTTGACCAACCAACGGGCCGAACTGCACGCGGCCGGCTGCACCAAG
CTGTTTTCCGAGAAGATCACCGGCACCAGGCGCGACCGCCCGGAGCTGGCCAGGATGCT
TGACCACCTACGCCCTGGCGACGTTGTGACAGTGACCAGGCTAGACCGCCTGGCCCGCA
GCACCCGCGACCTACTGGACATTGCCGAGCGCATCCAGGAGGCCGGCGCGGGCCTGCGT
AGCCTGGCAGAGCCGTGGGCCGACACCACCACGCCGGCCGGCCGCATGGTGTTGACCGT
GTTCGCCGGCATTGCCGAGTTCGAGCGTT