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WiscDsLox T-DNA Lines

There are two different varieties of WiscDsLox T-DNA Lines. 

1.  WiscDsLox

2.  WiscDsLoxHs

WiscDsLox and WiscDsLoxHs lines were made using completely different T-DNA vectors.  Click on the name of each line for detailed information.

Briefly:

1.  WiscDsLox lines carry a Ds transposon and LoxP sites.  No source of Ac Transposase is present in these lines.  WiscDsLox lines must be crossed to a line expressing Ac Transposase in order to mobilize the transposon. 

2.  WiscDsLoxHs lines carry a Ds transposon and LoxP sites.  In addition, these lines also carry a gene encoding Ac Transposase under the control of a heat-shock inducible promoter.  This vector was originally constructed by the laboratory of Venkatesan Sundaresan (Nishal et al, 2005).  We have modified Sundaresan's vector by introducing LoxP sites.