FISH Overview          

    The techniques described are taken from other people/papers please do not quote this manual as a direct reference.  The aim of this manual is to present the in situ hybridization methodology in a straightforward manner, describing “in-house secrets” that are usually omitted from published works.  We hope this manual will be particularly helpful to investigators setting up in situ hybridization de novo.  Following references are recommended for original information of the techniques.

References:

Sambrook, J., E.F. Fritsch and T. Maniatis (1989) Molecular cloning, a laboratory manual (second edition). (DNA isolation and purification).

Rayburn, A.L. and B.S. Gill (1985) Use of biotin-labeled probes to map specific DNA sequences on wheat chromosomes. J. Hered. 76: 78-81. (in situ hybridization)

Le, H.T., K.C. Armstrong, and B. Miki (1989) Detection of rye DNA in wheat-rye hybrids and wheat translocation stocks using total genomic DNA as a probe. Plant Mol. Biol. Rep. 7: 150-158. (Genomic in situ hybridization).

Schwarzacher, T., Leitch, A.R., Bennett, M.D., and Heslop-Harrison, J.S. (1989) In-situ localization of parental genomes in a wide hybrid. Ann. Bot. 64: 315-324. (Genomic in situ hybridization).

Leitch, I.J., A.R. Leitch, and J.S. Heslop-Harrison (1991) Physical mapping of plant DNA sequences by simultaneous in situ hybridization of two differently fluorescent probes.  Genome 34: 329-333.  (FISH, Multicolor FISH)

Jiang, J. and B.S. Gill (1993) Sequential chromosome banding and in situ hybridization analysis. Genome 36: 792-795. (Sequential chromosome banding and in situ hybridization).

Jiang, J., B.S. Gill, G.L. Wang, P.C. Ronald, and D.C. Ward (1995) Metaphase and interphase fluorescence in situ hybridization mapping of the rice genome with bacterial artificial chromosomes.  Proc. Natl. Acad. Sci. USA 92: 4487-4491.  (In situ hybridization using genomic DNA clones).